Don Antonio Osorio de Acuña was a Spanish bishop of Zamora, appointed 4 January 1507, during the reigns of Ferdinand II and Charles V. He filled that see in 1519, when the civil war broke out in Spain. Instead of espousing the interests of the throne, he joined the insurgent comuneros. According to his critics, his choice of allegiance was purely opportunistic and due to longstanding disputes with other members of the clergy.